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prolong gold antifade mounting medium without dapi  (Thermo Fisher)


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    Structured Review

    Thermo Fisher prolong gold antifade mounting medium without dapi
    Prolong Gold Antifade Mounting Medium Without Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prolong+gold+antifade+mounting+medium/prolong+diamond+antifade+mountant/pmc12271292-108-8-15
    Average 90 stars, based on 1 article reviews
    prolong gold antifade mounting medium without dapi - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Temporal and spatial niche partitioning in a retrotransposon community of the Drosophila melanogaster genome.
    Article Snippet: Embryos were transferred in PBS, 0.1% Tween (PBT), and 10% donkey serum, and either Donkey anti Rat Alexa 488 (Molecular Probes) or Donkey anti Guinea Pig Alexa 594 (Molecular Probes) was added at 1:500 dilution. .. After several washes in PBT and 4,6-diamidino-2phenylindole (DAPI) staining, embryos / ovaries were mounted in ProLong Gold Antifade mounting medium (Thermo Fisher Scientific). smiFISH coupled with vasa immunostaining was imaged with LSM 880 with SR Airyscan module (Zeiss) using ×40 / 1.4 NA objective. .. Airyscan processing was performed using 2D Zen Black v3.2 (Zeiss) prior to analysis. smiFISH coupled with traffic jam immunostaining was imaged with Leica SP8 confocal microscope equipped with ×40 / 1.4 NA objectives.

    Article Title: Temporal and spatial niche partitioning in a retrotransposon community of the Drosophila melanogaster genome
    Article Snippet: Embryos were transferred in PBS, 0.1% Tween (PBT), and 10% donkey serum, and either Donkey anti Rat Alexa 488 (Molecular Probes) or Donkey anti Guinea Pig Alexa 594 (Molecular Probes) was added at 1:500 dilution. .. After several washes in PBT and 4,6-diamidino-2-phenylindole (DAPI) staining, embryos/ovaries were mounted in ProLong Gold Antifade mounting medium (Thermo Fisher Scientific). smiFISH coupled with vasa immunostaining was imaged with LSM 880 with SR Airyscan module (Zeiss) using ×40/1.4 NA objective. .. Airyscan processing was performed using 2D Zen Black v3.2 (Zeiss) prior to analysis. smiFISH coupled with traffic jam immunostaining was imaged with Leica SP8 confocal microscope equipped with ×40/1.4 NA objectives.

    Immunostaining:

    Article Title: Temporal and spatial niche partitioning in a retrotransposon community of the Drosophila melanogaster genome.
    Article Snippet: Embryos were transferred in PBS, 0.1% Tween (PBT), and 10% donkey serum, and either Donkey anti Rat Alexa 488 (Molecular Probes) or Donkey anti Guinea Pig Alexa 594 (Molecular Probes) was added at 1:500 dilution. .. After several washes in PBT and 4,6-diamidino-2phenylindole (DAPI) staining, embryos / ovaries were mounted in ProLong Gold Antifade mounting medium (Thermo Fisher Scientific). smiFISH coupled with vasa immunostaining was imaged with LSM 880 with SR Airyscan module (Zeiss) using ×40 / 1.4 NA objective. .. Airyscan processing was performed using 2D Zen Black v3.2 (Zeiss) prior to analysis. smiFISH coupled with traffic jam immunostaining was imaged with Leica SP8 confocal microscope equipped with ×40 / 1.4 NA objectives.

    Article Title: Temporal and spatial niche partitioning in a retrotransposon community of the Drosophila melanogaster genome
    Article Snippet: Embryos were transferred in PBS, 0.1% Tween (PBT), and 10% donkey serum, and either Donkey anti Rat Alexa 488 (Molecular Probes) or Donkey anti Guinea Pig Alexa 594 (Molecular Probes) was added at 1:500 dilution. .. After several washes in PBT and 4,6-diamidino-2-phenylindole (DAPI) staining, embryos/ovaries were mounted in ProLong Gold Antifade mounting medium (Thermo Fisher Scientific). smiFISH coupled with vasa immunostaining was imaged with LSM 880 with SR Airyscan module (Zeiss) using ×40/1.4 NA objective. .. Airyscan processing was performed using 2D Zen Black v3.2 (Zeiss) prior to analysis. smiFISH coupled with traffic jam immunostaining was imaged with Leica SP8 confocal microscope equipped with ×40/1.4 NA objectives.

    Microscopy:

    Article Title: Novel non-coding FOXP3 transcript isoform associated to potential transcriptional interference in human regulatory T cells.
    Article Snippet: .. Finally, the slides were mounted using ProLong Gold antifade mounting medium (ThermoFisher Scientific) and kept at 4°C in the dark until acquisition on the confocal microscope. .. All RNAScope stainings were acquired at high resolution (104 nm) with a Zeiss LSM-880 confocal microscope using a 63× objective.

    Article Title: Hemodynamic forces prevent myxomatous valve disease in mice through KLF2/4 signaling
    Article Snippet: For p-SMAD2 staining, ImmPress TSA-based amplification was used at a dilution of 1:100 for 6 minutes (Akoya Bioscience). .. All sections were mounted with ProLong Gold Antifade mounting medium (Thermo Fisher Scientific, P36930 ), and imaging was performed on an Olympus BX53 Microscope. .. Quantification of microscopy images was performed using ImageJ v2.0 (NIH), including calculation of valve leaflet area and percentage area stained and counting of cells.

    Article Title: A major ecological niche of eosinophils in evolving Schistosoma granulomas challenges the eosinophil view as “helminth killer” cells
    Article Snippet: After washing with PBS and sequential blocking with 3% and 1% BSA, sections were incubated for 2 hours at RT with the secondary antibody donkey anti-sheep Alexa Fluor 594 (Thermo Fisher Scientific, catalog no. A-21098) at 5 μg/ml in 1% BSA (1:400 dilution). .. Sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific, catalog no. 62248) for visualization of nuclei and mounted using ProLong Gold Antifade mounting medium (Thermo Fisher Scientific, catalog no. P36934 ) for visualization under a fluorescence microscope (BX-60, Olympus, Melville, NY, USA). ..

    Imaging:

    Article Title: Hemodynamic forces prevent myxomatous valve disease in mice through KLF2/4 signaling
    Article Snippet: For p-SMAD2 staining, ImmPress TSA-based amplification was used at a dilution of 1:100 for 6 minutes (Akoya Bioscience). .. All sections were mounted with ProLong Gold Antifade mounting medium (Thermo Fisher Scientific, P36930 ), and imaging was performed on an Olympus BX53 Microscope. .. Quantification of microscopy images was performed using ImageJ v2.0 (NIH), including calculation of valve leaflet area and percentage area stained and counting of cells.

    other:

    Article Title: Hemodynamic forces prevent myxomatous valve disease in mice through KLF2/4 signaling
    Article Snippet: Sections were dewaxed with xylenes and rehydrated with decreasing concentrations of ethanol before staining with H&E (Abcam, ab245880) or Movat’s pentachrome (Abcam, ab245884).

    Fluorescence:

    Article Title: A major ecological niche of eosinophils in evolving Schistosoma granulomas challenges the eosinophil view as “helminth killer” cells
    Article Snippet: After washing with PBS and sequential blocking with 3% and 1% BSA, sections were incubated for 2 hours at RT with the secondary antibody donkey anti-sheep Alexa Fluor 594 (Thermo Fisher Scientific, catalog no. A-21098) at 5 μg/ml in 1% BSA (1:400 dilution). .. Sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific, catalog no. 62248) for visualization of nuclei and mounted using ProLong Gold Antifade mounting medium (Thermo Fisher Scientific, catalog no. P36934 ) for visualization under a fluorescence microscope (BX-60, Olympus, Melville, NY, USA). ..



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    Image Search Results


    a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .

    Journal: bioRxiv

    Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

    doi: 10.64898/2025.12.04.692390

    Figure Lengend Snippet: a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .

    Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

    Techniques:

    a, Representative immunofluorescent images of BODIPY + neutral lipids and GFAP + astrocyte in the hippocampal DG of PS19/E4 and PS19/E3 mice at 10 months of age. b-e, Correlations between BODIPY + neutral lipids in DG (% area) and Iba1 + % area ( n = 42) ( b ), AT8 + % area ( n = 31) ( c ), NeuN + % area ( n = 39) ( d ), and hippocampal volume ( n = 39) ( e ) in PS19/E3 mice, assessed via Pearson’s correlation analysis (two-sided). f, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of PS19/E4 mice at 4.0, 6.2, 7.8, and 10.0 months of age. Scale bars, 25μm. g, Correlation between age (in months) and BODIPY + neutral lipids in the hippocampal DG of PS19/E4 mice ( n = 20). Nonlinear regression R 2 . h-j, Representative immunofluorescent images of Rab5 + early endosomes (red, h ), Rab7 + late endosomes (red, i ), and LAMP1 + lysosomes (red, j ) with Iba1 + microglia (blue) and BODIPY + neutral lipid (green) in PS19/E4 mice. All scale bars, 40μm. k, Quantification of BODIPY + marker inside LAMP1 + stain ( n = 15).

    Journal: bioRxiv

    Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

    doi: 10.64898/2025.12.04.692390

    Figure Lengend Snippet: a, Representative immunofluorescent images of BODIPY + neutral lipids and GFAP + astrocyte in the hippocampal DG of PS19/E4 and PS19/E3 mice at 10 months of age. b-e, Correlations between BODIPY + neutral lipids in DG (% area) and Iba1 + % area ( n = 42) ( b ), AT8 + % area ( n = 31) ( c ), NeuN + % area ( n = 39) ( d ), and hippocampal volume ( n = 39) ( e ) in PS19/E3 mice, assessed via Pearson’s correlation analysis (two-sided). f, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of PS19/E4 mice at 4.0, 6.2, 7.8, and 10.0 months of age. Scale bars, 25μm. g, Correlation between age (in months) and BODIPY + neutral lipids in the hippocampal DG of PS19/E4 mice ( n = 20). Nonlinear regression R 2 . h-j, Representative immunofluorescent images of Rab5 + early endosomes (red, h ), Rab7 + late endosomes (red, i ), and LAMP1 + lysosomes (red, j ) with Iba1 + microglia (blue) and BODIPY + neutral lipid (green) in PS19/E4 mice. All scale bars, 40μm. k, Quantification of BODIPY + marker inside LAMP1 + stain ( n = 15).

    Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

    Techniques: Marker, Staining

    a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG mask. Quantified data in b-e represented as mean ± s.e.m., unpaired two-sided t-test. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/NSE-E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm. k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. Quantified data in k and l are represented as mean ± s.e.m., one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparisons test. For all experiments, PS19/E4, n = 35; PS19/E3 n = 42; PS19/E4/Syn1-Cre n = 29; and PS19/NSE-E4 n = 18. Pearson’s correlation analysis (two-sided) used for f-i .

    Journal: bioRxiv

    Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

    doi: 10.64898/2025.12.04.692390

    Figure Lengend Snippet: a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG mask. Quantified data in b-e represented as mean ± s.e.m., unpaired two-sided t-test. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/NSE-E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm. k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. Quantified data in k and l are represented as mean ± s.e.m., one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparisons test. For all experiments, PS19/E4, n = 35; PS19/E3 n = 42; PS19/E4/Syn1-Cre n = 29; and PS19/NSE-E4 n = 18. Pearson’s correlation analysis (two-sided) used for f-i .

    Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

    Techniques: